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KMID : 0380620120440030263
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Korean Journal of Food Science and Technology
2012 Volume.44 No. 3 p.263 ~ p.268
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Establishment of an Analytical Method for Novobiocin in Livestock Products Using HPLC-UVD
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Park Hee-Ra
Kwon Chan-Hyeok
Lee Jong-Goo
Kim Hyung-Soo
Chae Young-Sik
Oh Jae-Ho
Kwon Ki-Sung
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Abstract
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Novobiocin is a coumarin-containing antibiotic, and has a longer half-life in various animals than other veterinary medicines. A simple and rapid high-performance liquid chromatography assay for the determination of residual novobiocin levels in chicken, beef and milk has been developed and validated. The separation condition for HPLC/UVD was optimized by a MG II C18 (4.6 mm ID×250 mm, 5 μm) column with 0.1% formic acid in H2O/0.1% formic acid in Acetonitrile (40/60, v/v) as the mobile phase at a flow rate of 1.0 mL/min and the detection wavelength was set at 340 nm. Residues were extracted from tissue by blending with methanol. After liquid-liquid partitioning, lipid materials were removed with n-hexane and purification as Silica (1 g, 6 mL) cartridge with 10 mL acetone/dichloromethane (10/90, v/v). Limit of quantification and linearity performed by the analytical method were 0.02 mg/kg and 0.999 (r2), and the recovery range was 88.8±5.6-100.3±4.4, 88.8±7.2-97.0±3.2 and 88.1±4.3-92.8±3.6%. It is expected that this analytical method with regards to novobiocin in chicken, beef and milk could be applied as an official method to administer food safety on veterinary medicines.
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KEYWORD
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novobiocin, chicken, beef, milk, HPLC/UVD
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